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Main Title Aneuploidy Test Development: Kinetochore Staining in Mammalian Systems.
Author Tice, R. R. ; Dellarco, V. L. ;
CORP Author Brookhaven National Lab., Upton, NY.;Environmental Protection Agency, Washington, DC. Office of Health and Environmental Assessment.
Year Published 1986
Report Number EPA/600/8-86/006;
Stock Number PB86-163334
Additional Subjects Antibodies ; Feasibility studies ; Chromosomes ; Cells(Biology) ; In vitro analysis ; In vivo analysis ; Kinetochore ; Aneuploidy ; Cytogenetics ; Immunofluorescence staining
Library Call Number Additional Info Location Last
NTIS  PB86-163334 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 26p
Abstract The purpose of the project was to determine the feasibility of using human-derived antibodies against the chromosomal kinetochore region coupled with immunofluorescence staining as a method for evaluating the induction of aneuploidy in mammalian cells in vitro and in vivo. The technique was applied to (1) Chinese hamster cells (V79 cells), human fibroblasts, and peripheral lymphocytes interphase cells in vitro; (2) mouse bone marrow cells in vivo; and (3) mature human and rat sperm. Although kinetochore staining can be accomplished on a routine basis on mammalian interphase in vitro and in vivo cells, the technique does not appear to offer the staining intensity and/or persistence to allow for an accurate enumeration of the total genomic complement of a Chinese hamster, mouse or human cell. It was found that kinetochore structures are not visible in nature before or after techniques to cause sperm head enlargement. Kinetochore structures can, most likely, be detected in micronuclei.
Supplementary Notes Sponsored by Environmental Protection Agency, Washington, DC. Office of Health and Environmental Assessment.
NTIS Title Notes Rept. for Nov 83-Nov 84.
PUB Date Free Form Feb 86
Category Codes 6C; 57F
NTIS Prices PC A03/MF A01
Primary Description 600/21
Document Type NT
Cataloging Source NTIS/MT
Control Number 612915578
Origin NTIS
Type CAT