| Main Title |
Development and Validation of the Spiral Salmonella Assay: An Automated Approach to Bacterial Mutagenicity Testing. |
| Author |
Houk, V. S. ;
Schalkowsky, S. ;
Claxton., L. D. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Spiral System Instruments, Inc., Bethesda, MD. |
| Publisher |
c1989 |
| Year Published |
1989 |
| Report Number |
EPA/600/J-89/212; |
| Stock Number |
PB90-146184 |
| Additional Subjects |
Toxicity ;
Salmonella typhimuriun ;
Mutagens ;
Cyclophosphamide ;
Sodium azides ;
Test methods ;
Reprints ;
Mutagenicity tests ;
Dose-response relationships
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB90-146184 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
18p |
| Abstract |
Several automated approaches to Salmonella mutagenicity testing have been proposed in recent years but have failed to gain acceptance in the scientific community due to poor performance or lack of demonstrated usefulness. The paper reports on an automated system that successfully generates dose-response data and, moreover, reduces the labor, materials, and sample mass required to obtain such information. In the standard Salmonella plate-incorporation assay, dose-response relationships are defined by testing discrete doses of the test agent on a series of agar plates. In contrast, the spiral Salmonella assay generates dose-response data from a continuous concentration gradient on a single agar plate. Upon analysis, each spiral plate yields a dose-response curve consisting of 13 data points that span a concentration range of about 15:1. The performance of the spiral Salmonella assay was compared to that of the conventional plate-incorporation assay using 13 mutagens and 7 nonmutagens selected from a variety of chemical classes. |
| Supplementary Notes |
Pub. in Mutation Research, v223 p49-64 1989. Prepared in cooperation with Spiral System Instruments, Inc., Bethesda, MD. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Development and Validation of the Spiral Salmonella Assay: An Automated Approach to Bacterial Mutagenicity Testing. |
| Category Codes |
57Y; 57F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
005324106 |
| Origin |
NTIS |
| Type |
CAT |
