||Evidence for a Novel Pathway in the Degradation of Fluorene by 'Pseudomonas' sp. Strain F274.
Grifoll, M. ;
Selifonov, S. A. ;
Chapman, P. J. ;
||Environmental Research Lab., Gulf Breeze, FL. ;Barcelona Univ. (Spain). Dept. of Microbiology. ;University of West Florida, Pensacola. Center for Environmental Diagnostics and Bioremediation.
Soil pollution ;
Soil microbiology ;
Polycyclic aromatic hydrocarbons ;
Toxic substances ;
Pseudomonas sp strain F274 ;
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||A fluorene-utilizing microorganism, identified as a species of Pseudomonas, was isolated from soil severely contaminated from creosote use and was shown to accumulate six major metabolites from fluorene in washed-cell incubations. Five of these products were identified as 9-fluorenol, 9-fluorenone, (+)-1,1a-dihydroxy-1-hydro-9-fluorenone, 8-hydroxy-3,4-benzocoumarin, and phthalic acid. This last compound was also identified in growing cultures supported by fluorene. Fluorene assimulation into cell biomass was estimated to be approximately 50%. The structures of accumulated products indicate that a previously undescribed pathway of fluorene catabolism is employed by Pseudomonas sp. strain F274. On the basis of these findings, a pathway of fluorene degradation is proposed to account for its conversion to intermediary metabolites. A range of compounds with structures similar to that of fluorene was acted on by fluorene-grown cells to give products consistent with the initial reactions proposed.
||Pub. in Applied and Environmental Microbiology, v60 n7 p2438-2449 Jul 94. Also pub. as Environmental Research Lab., Gulf Breeze, FL. rept. no. CONTRIB-873. Prepared in cooperation with Barcelona Univ. (Spain). Dept. of Microbiology. and University of West Florida, Pensacola. Center for Environmental Diagnostics and Bioremediation.
|NTIS Title Notes
||Reprint: Evidence for a Novel Pathway in the Degradation of Fluorene by 'Pseudomonas' sp. Strain F274.
||68C; 68; 57K
||PC A03/MF A01