Main Title |
Evidence for a Novel Pathway in the Degradation of Fluorene by 'Pseudomonas' sp. Strain F274. |
Author |
Grifoll, M. ;
Selifonov, S. A. ;
Chapman, P. J. ;
|
CORP Author |
Environmental Research Lab., Gulf Breeze, FL. ;Barcelona Univ. (Spain). Dept. of Microbiology. ;University of West Florida, Pensacola. Center for Environmental Diagnostics and Bioremediation. |
Publisher |
c1994 |
Year Published |
1994 |
Report Number |
EPA/600/J-94/439; |
Stock Number |
PB95-112207 |
Additional Subjects |
Fluorene ;
Catabolism ;
Soil pollution ;
Reprints ;
Pseudomonas ;
Biodegradation ;
Soil microbiology ;
Polycyclic aromatic hydrocarbons ;
Toxic substances ;
Metabolites ;
Pseudomonas sp strain F274 ;
Microbial degradation
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB95-112207 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
13p |
Abstract |
A fluorene-utilizing microorganism, identified as a species of Pseudomonas, was isolated from soil severely contaminated from creosote use and was shown to accumulate six major metabolites from fluorene in washed-cell incubations. Five of these products were identified as 9-fluorenol, 9-fluorenone, (+)-1,1a-dihydroxy-1-hydro-9-fluorenone, 8-hydroxy-3,4-benzocoumarin, and phthalic acid. This last compound was also identified in growing cultures supported by fluorene. Fluorene assimulation into cell biomass was estimated to be approximately 50%. The structures of accumulated products indicate that a previously undescribed pathway of fluorene catabolism is employed by Pseudomonas sp. strain F274. On the basis of these findings, a pathway of fluorene degradation is proposed to account for its conversion to intermediary metabolites. A range of compounds with structures similar to that of fluorene was acted on by fluorene-grown cells to give products consistent with the initial reactions proposed. |
Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v60 n7 p2438-2449 Jul 94. Also pub. as Environmental Research Lab., Gulf Breeze, FL. rept. no. CONTRIB-873. Prepared in cooperation with Barcelona Univ. (Spain). Dept. of Microbiology. and University of West Florida, Pensacola. Center for Environmental Diagnostics and Bioremediation. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Evidence for a Novel Pathway in the Degradation of Fluorene by 'Pseudomonas' sp. Strain F274. |
Category Codes |
68C; 68; 57K |
NTIS Prices |
PC A03/MF A01 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
433518124 |
Origin |
NTIS |
Type |
CAT |