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Main Title Immunocytochemistry A Practical Guide for Biomedical Research / [electronic resource] :
Type EBOOK
Author Burry, Richard W.
Publisher Springer New York,
Year Published 2010
Call Number QD415-436
ISBN 9781441913043
Subjects Life sciences ; Immunology ; Biochemistry ; Cytology ; Microscopy
Internet Access
Description Access URL
http://dx.doi.org/10.1007/978-1-4419-1304-3
Collation XV, 223 p. online resource.
Notes Due to license restrictions, this resource is available to EPA employees and authorized contractors only
Contents Notes Antibodies -- Sample Preparation/Fixation -- Tissue Sectioning -- Blocking and Permeability -- Labels for Antibodies -- Application Methods -- Controls -- Method and Label Decision -- Single Antibody Procedure -- Multiple Antibodies Different Species -- Multiple Antibodies from the Same Species -- Fluorescent Microscopy and Imaging -- Troubleshooting -- Electron Microscopic Immunocytochemistry. Immunocytochemistry is a microscopic method that uses antibodies to localize proteins in tissue sections and cell cultures. Dr. Burry's book is the first practical guide to planning, performing, and evaluating immunocytochemical experiments designed for novice immunocytochemists. This book's predominant orientation is academic, focusing only on the most productive methods, with a primary emphasis on animal-based research tissues. The clear descriptions of the methods are easily followed and the specific design charts show how to plan an experiment. Included are a series of experimental cases exploring actual mistakes that demonstrate the approaches presented. Immunocytochemistry is no longer considered a specialized technique - Immunocytochemistry: A Practical Guide for Biomedical Research showcases this mainstream morphological approach in an accessible and exciting manner.
Place Published New York, NY
Corporate Au Added Ent SpringerLink (Online service)
Host Item Entry Springer eBooks
PUB Date Free Form 2010
BIB Level m
Medium computer
Content text
Carrier online resource
Cataloging Source OCLC/T
OCLC Time Stamp 20140912022203
Language eng
Origin SPRINGER
Type EBOOK
OCLC Rec Leader 02834nam a22005055i 45