| Main Title |
Mutagenicity and Clastogenicity of Teniposide (VM-26) in L5178Y/TK(+/-)-3.7.2C Mouse Lymphoma Cells. |
| Author |
DeMarini, D. M. ;
Brock, K. H. ;
Doerr, C. L. ;
Moore, M. M. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. |
| Year Published |
1987 |
| Report Number |
EPA/600/J-87/196; |
| Stock Number |
PB88-165980 |
| Additional Subjects |
Toxicology ;
Mice ;
Cell cultures ;
Reprints ;
Mutagenesis ;
Clastogenesis ;
Lymphoma ;
Teniposide
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB88-165980 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
The antitumor drug teniposide (VM-26) is a potent inducer of DNA breaks (Long et al., Cancer Res., 45:3106, 1985), but it is only weakly mutagenic at the hprt locus in CHO cells (Singh and Gupta, Cancer Res., 43:577, 1983). In the present study, the mutagenic and clastogenic activities of teniposide were evaluated in L5178Y/TK+/- -3.7.2C mouse lymphoma cells. While not a good mutagen at the hprt locus, teniposide is a potent mutagen at the tk locus, with as little as 0.5 ng/ml producing 220 TK mutants/ten to the 6th power survivors at 96% survival (background = 100/ten to the 6th power survivors). This same dose of teniposide induced 38 aberrations per 100 metaphases (background = 7/100 cells). At 7 ng/ml, teniposide induced approximately 2,700 TK mutants/ten to the 6th power survivors at approximately 10% survival. At the highest dose sampled for aberration analysis (5 ng/ml), teniposide induced 244 aberrations/100 cells. Most of the aberrations were chromosomal rather than chromatid events. As expected for a compound acting primarily by a clastogenic mechanism, most of the TK mutants were small colonies. |
| Supplementary Notes |
Pub. in Mutation Research, v187 p141-149 1987. Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Mutagenicity and Clastogenicity of Teniposide (VM-26) in L5178Y/TK(+/-)-3.7.2C Mouse Lymphoma Cells. |
| PUB Date Free Form |
1987 |
| Category Codes |
57Y; 57O |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
811323894 |
| Origin |
NTIS |
| Type |
CAT |
