| Main Title |
Large Deletions are Tolerated at the 'hprt' Locus of In vivo Derived Human T-Lymphocytes. |
| Author |
Fuscoe, J. C. ;
Zimmerman, L. J. ;
Harrington-Brock, K. ;
Moore, M. M. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. |
| Publisher |
c1992 |
| Year Published |
1992 |
| Report Number |
EPA/600/J-92/423; |
| Stock Number |
PB93-141224 |
| Additional Subjects |
Mutagenesis ;
Hypoxanthine phosphoribosyltransferase ;
T-lymphocyte gene rearrangement ;
Chromosome deletion ;
Polymerase chain reaction ;
DNA mutational analysis ;
Mutations ;
Cultured cells ;
Clone cells ;
T-lymphocytes ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-141224 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
A cloning assay was used to recover hypoxanthine phosphoribosyltransferase (hprt) T-lymphocytes from adult human males. Analysis of crude cellular extracts by polymerase chain reactions (PCRs) demonstrated that 8% (18/218) of the hprt mutations were due to total deletion of the hprt gene. Fourteen of the 18 mutants were examined by PCR for the presence of flanking DNA to determine the extent of the deletions. The largest deletions were greater than 15 times the size of the hprt gene. |
| Supplementary Notes |
Pub. in Mutation Research, v283 n4 p255-262 Dec 92. Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Large Deletions are Tolerated at the 'hprt' Locus of In vivo Derived Human T-Lymphocytes. |
| Category Codes |
57F |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
307031299 |
| Origin |
NTIS |
| Type |
CAT |
