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Main Title Mitotic Stability of Transforming DNA Is Determined by Its Chromosomal Configuration in the Fungus 'Cochliobolus heterostrophus'.
Author Keller, N. P. ; Bergstrom, G. C. ; Yoder, O. C. ;
CORP Author New York State Coll. of Agriculture and Life Sciences, Ithaca. Dept. of Plant Pathology.;Corvallis Environmental Research Lab., OR.
Publisher c1991
Year Published 1991
Report Number EPA-R-813458; EPA/600/J-91/103;
Stock Number PB91-206961
Additional Subjects Mitosis ; Deoxyribonucleic acids ; Fungi ; Fungal chromosomes ; Plasmids ; Antibiotic resistance ; Hygromycin B ; Promotor regions(Genetics) ; Bacterial genes ; Nucleic acid hybridization ; Autoradiography ; Methylation ; Reprints ; Cochliobolus heterostrophus
Library Call Number Additional Info Location Last
NTIS  PB91-206961 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Abstract Cochliobolus heterostrophus was transformed with a plasmid (pH1S) containing a bacterial gene (hygB), which confers resistance to the antibiotic hygromycin B when under control of an 838-bp fragment of promoter 1 from C. heterostrophus. The plasmid integrated at either homologous (52% single copy, 33% tandemly repeated copies) or ectopic (4% single copy, 11% tandemly repeated copies) sites on different chromosomes, resulting in four distinct configurations of integrated DNA. All four configurations were highly stable during mitotic growth; virtually no loss of integrated DNA was detected after five subcultures on nonselective medium or after seven cycles of pathogenesis on maize, the normal host of this fungus. However, deletion of integrated DNA was detected after eight or more disease cycles. (Copyright (c) Springer-Verlag 1991.)
Supplementary Notes Pub. in Current Genetics, v19 p227-233 1991. Sponsored by Corvallis Environmental Research Lab., OR.
NTIS Title Notes Journal article.
Title Annotations Reprint: Mitotic Stability of Transforming DNA is Determined by Its Chromosomal Configuration in the Fungus 'Cochliobolus heterostrophus'.
Category Codes 57F; 57K
NTIS Prices PC A02/MF A01
Primary Description 600/02
Document Type NT
Cataloging Source NTIS/MT
Control Number 124123242
Origin NTIS
Type CAT