| Main Title |
DNA Sequence Analysis of Revertants of the 'hisD3052' Allele of 'Salmonella typhimurium' TA98 Using the Polymerase Chain Reaction and Direct Sequencing: Application to 1-Nitropyrene-Induced Revertants. |
| Author |
Bell, D. A. ;
Levine, J. G. ;
DeMarini., D. M. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
EPA/600/J-91/098; |
| Stock Number |
PB91-200329 |
| Additional Subjects |
Bacterial DNA ;
Salmonella typhimurium ;
Mutation ;
Polymerase chain reaction ;
Alleles ;
Base sequence ;
Metabolic activation ;
Single-stranded DNA ;
Nucleic acid conformation ;
Agar gel electrophoresis ;
Reprints ;
1-Nitropyrene
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-200329 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
The study used the polymerase chain reaction (PCR) to speed the processing of revertants of Salmonella typhimurium TA98 for DNA sequence analysis. Briefly, a crude DNA extract from a single colony was prepared and used in an asymmetric PCR to amplify a 228-bp fragment containing the hisD3052 mutation approximately in the center. Following ultra-filtration, the ssDNA was sequenced using an end-labeled probe and dideoxy sequencing. The most frequent mutation among the revertants was a -2 deletion of GC or CG within the sequence CGCGCGCG, which is upstream of the hisD3052 mutation. The deletion occurred in 38% (6/16) of the spontaneous (-S9) revertants and in 94% (15/16) of a set of 1-nitropyrene-induced revertants. Misalignment of complementary DNA strands within this repeat may account for this mutation, although the possible formation of Z-DNA within this region may also play a role. Other mutations, mostly deletions but also some complex mutations (insertions/deletions/substitutions), occurred within quasi-palindromic regions of DNA. The potential DNA secondary structures within such regions may mediate the templated production of some of these mutations. |
| Supplementary Notes |
Pub. in Mutation Research, v252 n1 p35-44 Feb 91. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: DNA Sequence Analysis of Revertants of the 'hisD3052' Allele of 'Salmonella typhimurium' TA98 Using the Polymerase Chain Reaction and Direct Sequencing: Application to 1-Nitropyrene-Induced Revertants. |
| Category Codes |
57K; 57F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
122131013 |
| Origin |
NTIS |
| Type |
CAT |
