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Main Title Flow Cytometric Analysis of Effects of 1,3-Dinitrobenzene on Rat Spermatogenesis.
Author Evenson, D. P. ; Janca, F. C. ; Jost, L. K. ; Baer, R. K. ; Karabinus, D. S. ;
CORP Author South Dakota State Univ., Brookings. Dept. of Chemistry.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA-R-810991; EPA/600/J-89/372;
Stock Number PB90-216136
Additional Subjects Rats ; Testis ; Deoxyribonucleic acids ; Ribonucleic acids ; Mutations ; Reprints ; Flow cytometry ; Spermatogenesis ; Dinitrobenzenes ; Cell nucleus ; Dose-response relationships ; Vas deferens ; Epididymis
Library Call Number Additional Info Location Last
NTIS  PB90-216136 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 20p
Abstract Exposure of 100-d old rats to 1.3-dinitrobenzene (m-DNB) at dosages up to 48 mg/kg resulted in disruption of spermatogenesis as measured by flow cytometry (FCM) of acridine orange-stained sperm and testis cells. One day (d 1) after a single exposure to 48 mg/kg m-DNB. FCM measurements of caput epididymal fluid cells demonstrated the presence of testicular germinal epithelial cells apparently sloughed off into the epididymis. Also, at d 1 after the same exposure, a decrease in pachytene spermatocytes was observed. By d 16 after exposure to 32 or 48 mg/kg, testicular damage was evidenced by an alteration of cell type ratios in FCM-analyzed populations of testicular cells. Extensive recovery of cell type ratios occurred by d 32. At d 16, dosages of 32 and 48 mg/kg caused alterations of sperm chromatin structure as determined by the flow cytometric sperm chromatin structure assay (SCSA); 48 mg/kg caused alterations at both d 16 and d 32. Exposure to m-DNB caused a dose response increase in percent sperm head morphology abnormalities (%ABN) assessed in cauda epididymal and vas sperm. (Copyright (c) 1989 by Hemisphere Publishing Corporation.)
Supplementary Notes Pub. in Jnl. of Toxicology and Environmental Health, v28 p81-98 Sep 89. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Flow Cytometric Analysis of Effects of 1,3-Dinitrobenzene on Rat Spermatogenesis.
Category Codes 57Y; 57S; 57F
NTIS Prices PC A03/MF A01
Primary Description 600/10
Document Type NT
Cataloging Source NTIS/MT
Control Number 018016086
Origin NTIS
Type CAT