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OLS Field Name OLS Field Data
Main Title Oncogene Alterations in In vitro Transformed Rat Tracheal Epithelial Cells.
Author Mass, M. J. ; Schorschinsky, N. S. ; Lasley, J. A. ; Beeman, D. K. ; Austin., S. J. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number EPA-68-02-4456; EPA/600/J-90/083;
Stock Number PB90-245432
Additional Subjects Trachea ; Epithelium ; Toxicology ; In vitro analysis ; Cells(Biology) ; Methylation ; Reprints ; Oncogenes ; Neoplastic cell transformation ; Ras genes ; Genetic transcription ; Gene amplification ; Restriction fragmet length polymorphism ; Northern blotting ; Base sequence ; Codon ; Benzo(a)pyrene ; Benzanthracene/dimethyl ; Transfection ; Southern blotting
Library Call Number Additional Info Location Last
NTIS  PB90-245432 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 12/03/1990
Collation 10p
Abstract Ten derivations of rat tracheal epithelial (RTE) cells, including normal cells, normal primary cultures, 7 tumorigenic cell lines and 1 non-tumorigenic cell line transformed in vitro by treatment with 7,12-dimethylbenz(a)anthracene (DMBA), benzo(a)pyrene and/or 12-O-tetradecanoylphorbol-13-acetate (TPA) were examined for oncogene alterations. The analyses included amplification, restriction length polymorphisms (RFLPs), and changes in the expression of RNA transcripts of Ha-ras, Ki-ras, and c-myc. Two DMBA-derived cell lines displayed RFLPs after Eco RI digestion and probing for Ki-ras. No abnormalities of Ha-ras were seen after digestion with Bam HI, Eco RI, or Hind III. No extra Pst I (Ha-ras codon 12, GGA to CGA) or Xba I (Ha-ras codon 61, CAA to CTA) sites were seen after probing for Ha-ras. In one cell line derived by DMBA-treatment, changes in the c-myc restriction pattern were seen after digestion with Bam HI and Hind III. Analysis of CCGG sequences by Hpa II/Msp I digestion indicated that Ki-ras was highly methylated, Ha-ras exhibited limited methylation, and c-myc CCGG sequences were not methylated. In addition, normal cells removed from tracheas and those in primary cultures displayed Hpa II digestion patterns that were distinguishable from those of transformed cell lines by the presence of an apparent extra methylation site. (Copyright (c) 1990 Elsevier Science Publishers B.V. (Biomedical Division).)
Supplementary Notes Pub. in Mutation Research Letters, v243 n4 p291-298 Apr 90. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Oncogene Alterations in In vitro Transformed Rat Tracheal Epithelial Cells.
Category Codes 57Y; 57F
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Cataloging Source NTIS/MT
Control Number 024227999
Origin NTIS
Type CAT