Main Title |
Effects of Developmental Stage and Tissue Type on Embryo/Fetal DNA Distributions and 5-Fluorouracil-Induced Cell-Cycle Perturbations. |
Author |
Elstein, K. H. ;
Zucker, R. M. ;
Andrews, J. E. ;
Ebron-McCoy, M. ;
Shuey, D. L. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;ManTech Environmental Technology, Inc., Research Triangle Park, NC. |
Publisher |
c1993 |
Year Published |
1993 |
Report Number |
EPA/600/J-94/075; |
Stock Number |
PB94-141439 |
Additional Subjects |
Cell cycle ;
Fluorouracil ;
Toxicology ;
Embryos ;
Fetus ;
Deoxyribonucleic acid ;
Development ;
Flow cytometry ;
Liver ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB94-141439 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
11p |
Abstract |
Cell-cycle analysis of nuclei obtained from the circulating erythroblasts (gestational day (GD) 11-16), livers (GD 14-19), and whole embryos (GD 10-13) or remaining (extrahepatic) tissues (GD 14-16) of rat embryos/fetuses revealed age- and tissue-dependent variations in the relative percentages of cells in the G0/G1, S, and G2/M phases of the cell cycle. To determine how these developmental and organ-specific cell-cycle variations affect toxic response, we sampled GD 11-13 embryos 6 hr after maternal administration of a teratogenic dose of 5-fluorouracil (5-FU), a thymidylate synthetase inhibitor that induces S-phase accumulation. The results indicate that, on a relative basis, the amount of induced S-phase accumulation in erythroblasts and whole embryos 6 hr postdosing increased with development. In contrast, a time course of hepatic cell-cycle distributions from GD 14 embryos after maternal dosing revealed that S-phase accumulations occurred at an earlier time, possibly as a consequence of a higher proliferative rate. These findings suggest that the extent of observed toxicant-induced cell-cycle perturbations depends on gestational age, tissue type, and the time of sampling. |
Supplementary Notes |
Pub. in Teratology, v48 n4 p355-363 Oct 93. Prepared in cooperation with ManTech Environmental Technology, Inc., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Effects of Developmental Stage and Tissue Type on Embryo/Fetal DNA Distributions and 5-Fluorouracil-Induced Cell-Cycle Perturbations. |
Category Codes |
57Y; 57F |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
407722530 |
Origin |
NTIS |
Type |
CAT |