||Mutation and Enhanced Virus Transformation of Cultured Hamster Cells by Exposure to Gaseous Ethylene Oxide.
Hatch, G. G. ;
Conklin, P. M. ;
Christensen, C. C. ;
Anderson, T. M. ;
Langenbach, R. ;
||Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
In vitro analysis ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Ethylene oxide is a classical mutagen and a carcinogen based on evidence from studies in experimental animals. It is widely distributed in industrial, research, hospital, and food environments. In an effort to explore the use of newly developed methods for exposing mammalian cells to gaseous or volatile mutagens/carcinogens, Chinese hamster V79 cells were treated for 2 hr with gaseous ethylene oxide, in sealed treatment chambers and assayed for survival and mutagenic response by analysis of induced resistance to 6-thioguanine or ouabain. Significant numbers of mutants were produced at both genetic markers by 1,250-7,500 ppm ethylene oxide. Similarly, primary Syrian hamster embryo cells were treated for 2 or 20 hr with gaseous ethylene oxide in sealed treatment chambers and subsequently assayed for survival and increased sensitivity to SA7 virus transformation. Treatment concentrations extended from toxic to several nontoxic concentrations.
||Pub. in Environmental Mutagenesis, v8 n1 p67-76 Jan 83. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
|NTIS Title Notes
||Reprint: Mutation and Enhanced Virus Transformation of Cultured Hamster Cells by Exposure to Gaseous Ethylene Oxide.
|PUB Date Free Form
||6T; 6C; 57Y; 57F
||PC A02/MF A01