Main Title |
Efficacy of beta-Glucuronidase Assay for Identification of 'Escherichia coli' by the Defined-Substrate Technology. |
Author |
Rice, E. W. ;
Allen, M. J. ;
Edberg, S. C. ;
|
CORP Author |
Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;American Water Works Association Research Foundation, Denver, CO. ;Yale Univ., New Haven, CT. Dept. of Lab. Medicine. |
Publisher |
c1990 |
Year Published |
1990 |
Report Number |
EPA/600/J-90/149; |
Stock Number |
PB91-109876 |
Additional Subjects |
Escherichia coli ;
Bioassay ;
Enzymes ;
Water pollution ;
Feces ;
Humans ;
Livestock ;
Reprints ;
Glucuronidase ;
Drinking water
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-109876 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
5p |
Abstract |
In 1976, Kilian and Bulow described the association of B-Glucuronidase with the genus Escherichia (97% positive) and suggested that a B-Glucuronidase assay would be a useful identification test. Since that report, papers about the sensitivity and specificity of this enzyme for the identification of Escherichia coli from clinical sources, food, seawater, potable-water supplies, and various environmental sources have appeared. A study was undertaken to determine the efficacy and specificity of the defined-substrate technology B-glucuronidase (Colilert) assay for the identification of this species from fecal samples. A total of 460 human, 105 cow, and 55 horse E. coli isolates were tested. Results showed 95.5% B-Glucuronidase-positive isolates in 24 h and 99.5% positive after 28 h of incubation. Only one E. coli isolate was negative. There were no significant differences in the percentage of B-Glucuronidase-positive isolates among the human or animal isolates. There were no non-E. coli isolates that were positive. All subjects carried B-Glucuronidase-positive E. coli. (Copyright (c) 1990 American Society for Microbiology.) |
Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v56 n5 p1203-1205 May 90. Prepared in cooperation with American Water Works Association Research Foundation, Denver, CO., and Yale Univ., New Haven, CT. Dept. of Lab. Medicine. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Efficacy of beta-Glucuronidase Assay for Identification of 'Escherichia coli' by the Defined-Substrate Technology. |
Category Codes |
68D; 57K |
NTIS Prices |
PC A01/MF A01 |
Primary Description |
600/14 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
034834779 |
Origin |
NTIS |
Type |
CAT |