OLS : Record


Main Title Application of DNA-DNA Colony Hybridization to the Detection of Catabolic Genotypes in Environmental Samples.
Author Sayler, G. S.; Shields, M. S.; Tedford, E. T.; Breen, A.; Hooper, S. W.;
CORP Author Tennessee Univ., Knoxville.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Year Published 1985
Report Number EPA/600/J-85/116;
Stock Number PB86-101839
Subjects Bacteria; Toluene; Naphthalene; Aromatic hydrocarbons; DNA; Hybridization; Chemical attack(Degradation); Environmental microbiology; Molecular cloning; Environmental pollutants; Plasmids
Library   Call Number Additional Info Location Date Modified
NTIS PB86-101839 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 10p
Abstract The application of preexisting DNA hybridization techniques was investigated for potential in determining populations of specific gene sequences in environmental samples. Cross-hybridizations among two degradative plasmids, TOL and NAH, and two cloning vehicles, pLAFRI and RSF1010, were determined. The detection limits for the TOL plasmid against a nonhomologous plasmid-bearing bacterial background was ascertained.
Availability Notes Pub. in Applied and Environmental Microbiology, v49 n5 p1295-1303 May 85.
NTIS Title Notes Journal article.
PUB Date Free Form c1985
Category Codes 6F; 6M; 57K; 57H; 57F
NTIS Prices Not available NTIS
Primary Description 600/15
Document Type NT
Control Number 535324861
Cataloging Source NTIS/MT
Origin NTIS
Type CAT