OLS : Record


Main Title Ammonia Removal from Mammalian Cell Culture Medium by Ion-Exchange Membranes.
Author Sikdar, S. K.; Sawant, S. B.;
CORP Author Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;Bombay Univ. (India). Dept. of Chemical Technology.
Publisher c1994
Year Published 1994
Report Number EPA/600/J-94/371;
Stock Number PB95-124863
Subjects Ammonia; Cultured cells; Culture media; Ion exchange resins; Artifical membrane; Lactic acid; Reprints; Perfluorosulfonic acid polymer membranes
Library   Call Number Additional Info Location Date Modified
NTIS PB95-124863 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/06/1995
Collation 13p
Abstract Metabolites such as ammonia and lactic acid formed during mammalian cell culture can frequently be toxic to the cells themselves beyond a threshold concentration of the metabolites. Cell culture conducted in the presence of such accumulated metabolites is therefore limited in productivity. This work demonstrates with laboratory data that a nonporous ion-exchange membrane of the perfluorinated sulfonic acid type can be used to contact the culture medium, and ammonia removed selectivity from the medium without disturbing the process. The technique of pervaporation showed particular promise in this regard. The pervaporation used with inert gas sweep on the permeate side was found superior to that used with vacuum application.
Supplementary Notes Pub. in Separation Science and Technology 29, n12 p1579-1591 1994. Prepared in cooperation with Bombay Univ. (India). Dept. of Chemical Technology.
NTIS Title Notes Journal article.
Title Annotations Reprint: Ammonia Removal from Mammalian Cell Culture Medium by Ion-Exchange Membranes.
Category Codes 57F; 99F
NTIS Prices PC A03/MF A01
Primary Description 600/14
Document Type NT
Control Number 435317630
Cataloging Source NTIS/MT
Origin NTIS
Type CAT