OLS : Record


Main Title A tissue enzyme assay for chlorinated hydrocarbon insecticides.
Author Cutkomp, L. K.
CORP Author Minnesota Univ., St. Paul. Dept. of Entomology, Fisheries and Wildlife.
Publisher For sale by the Supt. of Docs., U.S. Govt. Print. Off.,
Place Published Washington,
Year Published 1974
Report Number EPA-660/2-73-027; EPA-R-801029; EPA-16030-ELZ; W74-10526
Stock Number PB-234 404
OCLC Number 01213615
Subjects Chlorohydrocarbons; Insecticides; Acaricides; Lethal dosage; DDT; Pesticides; Assaying; Enzymes; Indicator species; Toxicology; Fishes; Brain; Phosphorus organic compounds; Carbamates; Physiological effects; Water pollution effects(Animals); Phosphatase/adenosine-tri
Subject Added Ent Insecticides--Toxicology.; Insecticides--Physiological effect.; Organochlorine compounds.; Enzymes--Analysis.; Adenosinetriphosphatase--deficiency.; Hydrocarbons, Chlorinated.; Insecticides--adverse effects.; Adenosine Triphosphatases--deficiency.; Insecticides, Organochlorine--adverse effects
Internet Access
Description Access URL
Library   Call Number Additional Info Location Date Modified
EJAD EPA 660/2-73-027 Region 3 Library/Philadelphia, PA 05/24/1996
EJBD ARCHIVE EPA 660-2-73-027 Headquarters Library/Washington,DC 01/12/2015
EJBD EPA 660-2-73-027 Headquarters Library/Washington,DC 01/12/2015
EKAM SB951.5.C88 Region 4 Library/Atlanta,GA 03/14/1997
ERAD EPA 660/2-73-027 Region 9 Library/San Francisco,CA 03/18/2013
NTIS PB-234 404 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 01/01/1988
Collation vi, 39 pages illustrations 27 cm.
Abstract Certain chlorinated hydrocarbon insecticides, especially DDT and closely related chemicals, tested at low concentrations, adversely affect the ATPase enzyme system. DDT inhibited oligomycin-sensitive Mg(2+)ATPase (mitochondrial) both in vitro and in vivo. About 1 micromolar (1 x 10 to the 6th power M) gave 50% inhibition in fish brain and 0.5 parts per billion of DDT in water inhibited about 50% of mitochondrial Mg(2+)ATPase. Na(+)-K(+)ATPase was not inhibited in brain, but was inhibited in vivo in fish gills. Certain discriminating effects were found among chlorinated hydrocarbons, particularly with respect to inhibition of Mg(2+)ATPase, but the ranking of compounds by enzymic effects does not always parallel toxicity values. Organophosphate and carbamate insecticides were ineffective. Further research is needed both in vitro and in vivo to determine how the adverse effects on the enzymes relate to practical interpretations of effects. The abnormally low ATPase activity in chronically treated fish is the first report of an adverse biochemical effect with sublethal doses of DDT.
Notes "Project 16030 ELZ; Program element 1BA027." Includes bibliographical references (pages 37-38).
Availability Notes Paper copy available from GPO $0.85.
Corporate Au Added Ent United States. Environmental Protection Agency. Office of Research and Development.
Title Ser Add Ent Research reporting series. Environmental protection technology ; EPA-660/2-73-027.
PUB Date Free Form 1974
Series Title Traced Environmental protection technology series, EPA-660/2-73-027
Series Title Untraced Environmental protection technology series, EPA-660/2-73-027
BIB Level m
Medium unmediated
OCLC TAG 19s 14302865; 14888637
OCLC Time Stamp 20150106080124
Cataloging Source OCLC/T
Language eng
SUDOCS Number EP 1.23/2:660/2-73-027
Origin OCLC
OCLC Rec Leader 02395cam 2200517I 45020