OLS : Record


Main Title Method 1103.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using Membrane-Thermotolerant Escherichia coli Agar (mTEC), March 2010.
CORP Author Environmental Protection Agency, Washington, DC. Office of Water.
Year Published 2010
Report Number EPA/821/R-10/002
Stock Number PB2010-114246
Subjects Escherichia coli; Membrane filtration; Water sampling; Bacteria; Agar; Enumeration; Epidemiology; Microorganisms; Criteria; Water quality; Marine environment; Health effects; Gastroenteristis; Feces; Water pollution effects; Surface waters; Counting methods; Calibration; Growth(Biology); Contamination; Quality control; Pollution prevention; Laboratories; Test methods
Library   Call Number Additional Info Location Date Modified
NTIS PB2010-114246 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 01/21/2011
Collation 46p
Abstract The MF method provides a direct count of bacteria in water based on the development of colonies on the surface of the membrane filter (Reference 18.4). A water sample is filtered through the membrane which retains the bacteria. After filtration, the membrane is placed on a selective and differential medium, mTEC, incubated at 35 degrees C + or = 0.5 degrees C for 2 + or = 0.5 hours to resuscitate injured or stressed bacteria, and then incubated at 44.5 degrees C + or = 0.2 degrees C for 22 + or = 2 hours. Following incubation, the filter is transferred to a filter pad saturated with urea substrate. After 15 minutes, yellow, yellow-green, or yellow-brown colonies are counted with the aid of a fluorescent lamp and a magnifying lens.
Supplementary Notes See also PB2007-101966, July 2006.
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PUB Date Free Form Mar 2010
Category Codes 57K; 57U; 68D
NTIS Prices PC A04
Document Type NT
Control Number 128601780
Cataloging Source NTIS/MT
Origin NTIS
Type CAT