Main Title |
Determination and Enhancement of Anaerobic Dehalogenation: Degradation of Chlorinated Organics in Aqueous Systems. |
Author |
Palmer, D. T. ;
Linkfield, T. G. ;
Robinson, J. B. ;
Sharak Genthner, B. R. ;
Pierce, G. E. ;
|
CORP Author |
Battelle Columbus Div., OH.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
Publisher |
Sep 88 |
Year Published |
1988 |
Report Number |
EPA/600/2-88/054; |
Stock Number |
PB89-110282 |
Additional Subjects |
Water pollution control ;
Chlorine organic compounds ;
Anaerobic bacteria ;
Water treatment ;
Performance evaluation ;
Chemical analysis ;
Bioengineering ;
Genetics ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB89-110282 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
59p |
Abstract |
The report summarizes initial efforts: to isolate microorganisms capable of anaerobic dehalogenation; to examine the nutritional requirements of dehalogenating enrichments and a dehalogenating consortium; and to study the genetics of dehalogenation. Enrichments using secondary anaerobic digestor sludge containing either 3- or 4-chlorobenzoate were established with a variety of terminal electron acceptors. Degradation of 4-chlorobenzoate was not observed. Degradation of 3-chlorobenzoate (3CB) was observed after 10-23 weeks. Use of a 3CB degrading consortium revealed that the microorganisms capable of benzoate degradation and methanogenesis were lost but dechlorination continued. Genetic studies using a pure culture of strain DCB-1 found the strain to be suitable for cloning manipulations. |