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RECORD NUMBER: 554 OF 5096

OLS Field Name OLS Field Data
Main Title Assessment of the Microscreen Phage-Induction Assay for Screening Hazardous Wastes (1989).
Author Houk, V. S. ; DeMarini., D. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher 1989
Year Published 1989
Report Number EPA/600/D-89/137;
Stock Number PB90-116179
Additional Subjects Hazardous wastes ; Toxicology ; Escherichia coli ; Salmonella typhimurium ; Tables(Data) ; Mutagenicity tests ; Bacteriophages ; Metabolic activation ; Volatile organic compounds ; Dose-response relationships
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB90-116179 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/10/1990
Collation 21p
Abstract
The Microscreen phage-induction assay, which quantitatively measures the induction of prophage Lambda in Escherichia coli WP2s(Lambda), was used to test 14 crude (unfractionated) hazardous industrial waste samples for genotoxic activity in the presence and absence of metabolic activation. Eleven of the 14 wastes induced prophage, and induction was observed at concentrations as low as 0.4 picograms per ml. Comparisons of the mutagenic activity of these waste samples in Salmonella and their ability to induce prophage Lambda indicate that the phage-induction assay was a more sensitive indicator of genetic damage for this group of wastes. All but one of the wastes that were mutagenic to Salmonella were detected by the phage-induction assay, and 5 wastes not mutagenic to Salmonella were genetically active in the phage assay. The enhanced ability of the phage-induction assay to detect genotoxic activity may be related to the constituents comprising these waste samples. Partial chemical characterizations of the wastes showed high concentrations of carcinogenic metals, solvents, and chlorinated compounds, most of which are detected poorly by the Salmonella assay.