Main Title |
Characterization of an Axenic Strain of 'Hartmannella vermiformis' Obtained from an Investigation of Nosocomial Legionellosis. |
Author |
Fields, B. S. ;
Nerad, T. A. ;
Sawyer, T. K. ;
King, C. H. ;
Barbaree, J. M. ;
|
CORP Author |
Center for Infectious Diseases, Atlanta, GA. Div. of Bacterial Diseases. ;American Type Culture Collection, Rockville, MD. ;Rescon Associates, Inc., Royal Oak, MD. ;Georgia Univ., Athens. Coll. of Veterinary Medicine.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
Publisher |
c1990 |
Year Published |
1990 |
Report Number |
EPA-R-816070-01-0; EPA/600/J-90/525; |
Stock Number |
PB91-211425 |
Additional Subjects |
Hartmanella ;
Cross infections ;
Legionellosis ;
Legionella ;
Isoenzymes ;
Water microbiology ;
Polyacrylamide gel electrophoresis ;
Reprints ;
Protozoan-bacterial interactions
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-211425 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
5p |
Abstract |
A free living amoeba identified as Hartmannella vermiformis was isolated from a water sample obtained during an investigation of nosocomial legionellosis. Hartmannella vermiformis are known to support the intracellular multiplication of Legionella pneumophila. This strain of H. vermiformis, designed CDC-19, was cloned and established in axenic culture, in order to develop a model for the study of the pathogenicity of legionellae. Isoenzyme patterns of axenically cultivated strain CDC-19 were compared with 2 strains of H. vermiformis derived from the type strain, one axenic (ATCC 50236) and the other grown in the presence of bacteria (ATCC 30966). Enzyme patterns suggested that all 3 strains are assignable to the species Hartmannella vermiformis. Axenic H. vermiformis strain CDC-19 has been deposited with the American Type Culture Collection (ATCC 50237) and should prove useful in the study of protozoan-bacterial interaction. (Copyright (c) 1990 American Society of Protozoologists.) |