||Determination of 1,2-Diols by Indirect Atomic Absorption with Digested Lead Periodate.
Tan, Barrie ;
Melius, Paul ;
Kilgore, Melvin V. ;
||Auburn Univ., AL. Dept. of Chemistry.;Environmental Research Lab., Gulf Breeze, FL.
||EPA-R-806213-010; EPA-600/J-80-079 ; ERL.GB-J0074
Chemical analysis ;
Atomic spectroscopy ;
Absorption spectra ;
Toxic substances ;
Biological samples ;
Atomic absorption spectrophotometry ;
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Techniques are described for the determination of 1,2-Diols by indirect atomic absorption with digested lead periodate. Preliminary experiments indicate that styrene glycol can be determined in the presence of styrene epoxide. This is of particular interest because epoxide hydrase catalyzes the hydrolysis of an epoxide to a 1,2-diol. The procedure may lend itself to the determination of the enzyme activity of epoxide hydrase, known for its pivotal role in the metabolism of many carcinogens and toxic compounds.