Record Display for the EPA National Library Catalog

RECORD NUMBER: 182 OF 1515

OLS Field Name OLS Field Data
Main Title Characterization of Subsurface Bacteria Associated with Two Shallow Aquifers in Oklahoma.
Author Balkwill, D. L. ; Ghiorse, W. C. ;
CORP Author Florida State Univ., Tallahassee. Dept. of Biological Science. ;Cornell Univ., Ithaca, NY.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Year Published 1985
Report Number EPA/600/J-85/203;
Stock Number PB86-117389
Additional Subjects Bacteria ; Aquifers ; Electron microscopy ; Cells(Biology) ; Counting methods ; Cell morphology ; Aerobic processes ; Arthrobactes ; Actiniomycetales ;
Holdings
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Status
NTIS  PB86-117389 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 11p
Abstract
The bacterial microflora of two shallow aquifers in Oklahoma was characterized by direct observation with light (LM) and electron microscopy (EM), by plating, and by examination of colony morphology and distribution. Total cell counts varied only slightly from sample to sample, whereas colony counts varied widely. Colony counts on nutritionally-rich (NR) media were lower than on low-nutrient (LN) media, especially in samples from the saturated zone. The variety of colony types growing on NR media depth decreased with depth. Cell morphologies of bacteria grown aerobically on plates included short rods, cocci, and actinomycete-like forms. Direct LM observation of sediments revealed short, rod-shaped and coccoid bacterial cells. EM observation of bacteria released from the samples revealed that 85-90% of them were coccoid, Gram-positive, Arthrobacter-like organisms some of which were dividing or contained completed division septa. (Copyright (c) 1985, American Society for Microbiology.)