Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title The ABCs of Gene Cloning [electronic resource] /
Author Wong, Dominic W. S.
Publisher Springer US,
Year Published 2006
Call Number QH431
ISBN 9780387286792
Subjects Medicine. ; Human genetics. ; Food science. ; Plant physiology. ; Zoology.
Internet Access
Description Access URL
Edition Second Edition.
Collation XVI, 248 p. online resource.
Due to license restrictions, this resource is available to EPA employees and authorized contractors only
Contents Notes
Fundamentals of Genetic Processes -- Introductory Concepts -- Structures of Nucleic Acids -- Structures of Proteins -- The Genetic Process -- Organization of Genes -- Reading The Nucleotide Sequence of a Gene -- Techniques and Strategies of Gene Cloning -- Enzymes Used in Cloning -- Techniques Used in Cloning -- Cloning Vectors for Introducing Genes into Host Cells -- Transformation -- Isolating Genes for Cloning -- Impact of Gene Cloning -- Improving Tomato Quality by Antisense Rna -- Transgenic Crops Engineered with Insecticidal Activity -- Transgenic Crops Conferred with Herbicide Resistance -- Growth Enhancement in Transgenic Fish -- Impact of Gene Cloning -- Microbial Production of Recombinant Human Insulin -- Finding Disease-Causing Genes -- Human Gene Therapy -- Gene Targeting -- DNA Typing -- Transpharmers - Bioreactors for Pharmaceutical Products -- Animal Cloning -- Human Genome Sequencing. The ABCs of Gene Cloning, Second Edition Dominic W. S. Wong The ABCs of Gene Cloning serves as a clear and easy-to-use text that offers the reader an introductory course on the language of gene cloning. It assumes no prior knowledge on the part of the reader, while emphasizing the 'nuts and bolts' of gene cloning. The ABCs of Gene Cloning enables the reader to distinguish between a gene and a DNA, to read and write a gene sequence, to talk intelligently about gene cloning, to attend seminars without getting completely lost, and to comprehend materials in scientific publications. The ABCs of Gene Cloning contains updated information throughout, a tutorial on how to read a gene sequence, new topics on animal cloning and human genome sequencing, and rewritten chapters on human gene therapy, gene targeting, and DNA typing. Part I of this book outlines the very basic principles of biology and genetics, with emphasis only on the aspects relevant to gene cloning. The intent is to provide sufficient background materials without burdening the reader with unwarranted details. Part II describes the common techniques and approaches of gene cloning used in microbial, plant and mammalian systems. It includes topics ranging from the basic mechanics of DNA manipulation, vector construction, to genomic analysis, which is still a very new and rapidly evolving field. Part III presents selected applications of major impact on biomedicine, agriculture and related areas. Case examples are used to demonstrate how gene cloning is conducted and applied in biomedicine and agriculture. Key features: Emphasizes the vocabulary and language of gene cloning. Introduces the essentials of genetic processes relevant to cloning. Explains how to read a gene sequence. Presents basic techniques and strategies of gene cloning. Focuses on topics of applications impacting biomedicine and other fields Clear, concise, and extensively illustrated. Revised with updated information, new topics, and rewritten chapters. The ABCs of Gene Cloning is essential reading for students in biological science, biomedicine, and related disciplines, as well as others who have an interest in gaining proficiency in the language of gene cloning. The book assumes no prior knowledge of the subject, and uniquely provides the nuts and bolts for learning the art of gene cloning in a simple, logical, and thorough manner. The Author: Dominic W. S. Wong, Ph.D. is a scientist at the Western Regional Research Center, Agricultural Research Service, U. S. Department of Agriculture. Dr. Wong is also the author of Mechanisms and Theory in Food Chemistry and Food Enzymes: Structure and Mechanism, and co-editor of Handbook of Food Enzymology.