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RECORD NUMBER: 12 OF 28

OLS Field Name OLS Field Data
Main Title Gene engineering of yeasts for the degradation of hazardous waste /
Author Loper, John C.
CORP Author Cincinnati Univ., OH. Coll. of Medicine.;Environmental Protection Agency, Cincinnati, OH. Hazardous Waste Engineering Research Lab.
Publisher U.S. Environmental Protection Agency, Hazardous Waste Engineering Research Laboratory,
Year Published 1988
Report Number EPA/600-M-88-001; Air Force no. ESL-TR-86-52; EPA-R-810605
Stock Number PB88-182597
OCLC Number 18396082
Subjects Hazardous wastes--Biodegradation. ; Yeast.
Additional Subjects Hazardous wastes ; Biodeterioration ; Yeasts ; Cytochromes ; Antibodies ; Laboratory animals ; Deoxyribonucleic acids ; Genetic intervention
Internet Access
Description Access URL
https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=300024GD.PDF
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
EHAM  EPA/600/M-88/001 Region 1 Library/Boston,MA 05/25/2016
EJBD ARCHIVE EPA 600-M-88-001 In Binder Headquarters Library/Washington,DC 02/15/2017
EJBD  EPA 600-M-88-001 Headquarters Library/Washington,DC 07/19/2017
EKAM  EPA/600/M-88/001 Region 4 Library/Atlanta,GA 03/28/1998
ELBD RPS EPA 600-M-88-001 repository copy AWBERC Library/Cincinnati,OH 10/17/2014
NTIS  PB88-182597 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 01/01/1988
Collation 3 pages ; 28 cm
Abstract
The research examined the structure and function of cytochrome P-450 genes in yeast as a model for gene engineering such as eukaryotic P-450 enzymes for biodegradation of hazardous waste by yeasts. Saccharomyces cerevisiae and Candida tropicalis are two yeasts known to produce major P-450 enzymes. These enzymes were purified and antibodies produced in rabbits were then used in the isolation or characterization of clones containing a P-450 gene from each organism. DNA sequence was determined for the gene isolated from S. cerevisiae and for several hundred bases of chromosomal DNA on each side of the gene, and deletion experiments in the promoter region were conducted. The deduced protein sequence from this gene was compared to those of the other known subfamilies of eukaryotic P-450 genes; the several features identified included a new homology region for these protein sequences. The C. tropicalis gene and its promoter region have been partially sequenced.
Notes
Caption title. At head of title: Environmental research brief. Shipping list no.: 88-499-P. "Jan. 1988." "EPA/600-M-88-001." "Air Force No. ESL-TR-86-52."