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RECORD NUMBER: 9 OF 11

OLS Field Name OLS Field Data
Main Title Quantification of Glial Fibrillary Acidic Protein: Comparison of Slot-Immunobinding Assays with a Novel Sandwich ELISA.
Author O'Gallaghan, J. P. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-92/252;
Stock Number PB92-206325
Additional Subjects Glial fibrillary acidic protein ; Chemical analysis ; Toxicology ; Enzyme-linked immunosorbent assay ; Central nervous system ; Rats ; Trimethyltin ; Western blotting ; Reprints ; Methylphenyltetrahydropyridines
Holdings
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Status
NTIS  PB92-206325 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/01/1993
Collation 9p
Abstract
Detailed protocols are presented for assaying glial fibrillary acidic protein (GFAP), an astrocyte localized protein which serves as a quantitative marker of toxicant-induced injury to the central nervous system. Two different solid-phase assay procedures are described: (1) a nitrocellulose based slot-immunobinding assay and (2) a novel microtiter plate based sandwich ELISA. The performance of both assays was assessed by measuring the content of GFAP in homogenates of specific regions of the rat brain and in homogenates of brain regions damaged by the prototype neurotoxicants, trimethyltin (TMT) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Both procedures gave similar results that were consistent with previously published observations. By comparing the simplicity, cost effectiveness, safety and speed of the two methods, it appears likely that the sandwich ELISA has several advantages over slot-immunobinding assays.