Record Display for the EPA National Library Catalog

RECORD NUMBER: 3 OF 11

OLS Field Name OLS Field Data
Main Title Disruption of the 'Saccharomyces cerevisiae' Gene for NADPH-Cytochrome P450 Reductase Causes Increased Sensitivity to Ketoconazole.
Author Sutter, T. R. ; Loper, J. C. ;
CORP Author Cincinnati Univ., OH. Coll. of Medicine.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1989
Year Published 1989
Report Number EPA-CR-813366; EPA/600/J-93/305;
Stock Number PB93-229276
Additional Subjects Fungal genes ; NADH dehydrogenase ; Saccharomyces cerevisiae ; Ketoconazole ; Microbial sensitivity tests ; Plasmids ; Genetic complementation tests ; Southern blotting ; Western blotting ; Reprints ; Phenotype ; Candida tropicalis
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB93-229276 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/22/1993
Collation 12p
Abstract
Strains of Saccharomyces cerevisiae deleted in the NADPH-cytochrome P450 reductase gene by transplacement are 200-fold more sensitive to ketoconazole, an inhibitor of the cytochrome P450 lanosterol 14alpha-demethylase. Resistance is restored through complementation by the plasmid-borne wild type gene from either S. cerevisiae or Candida tropicalis. Neither Southern hybridization nor Western immunoblot techniques provided evidence for a second NADPH-cytochrome P450 reductase gene, suggesting that an alternate pathway may provide for the functions of this reductase in S. cerevisiae. (Copyright (c) 1989 Academic Press, Inc.)