Record Display for the EPA National Library Catalog

RECORD NUMBER: 27 OF 28

OLS Field Name OLS Field Data
Main Title Type A Viral Hepatitis: Effect of Chlorine on Infectivity.
Author Peterson, David A. ;
CORP Author Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL.;Municipal Environmental Research Lab., Cincinnati, OH.
Year Published 1982
Report Number EPA-R-805986; EPA-600/2-82-089;
Stock Number PB83-115170
Additional Subjects Viruses ; Chlorination ; Disinfection ; Water treatment chemicals ; Monkeys ; Laboratory animals ; Microorganism control(Water) ; Potable water ; Toxicity ; Hepatitis A viruses ; Infectivity ; Marmosets ; Pathogens ; Drinking water ; Water pollution control
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB83-115170 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 30p
Abstract
The objective of this study was to determine the effect of (HOCl) treatment on the infectivity of hepatitis A virus (HAV). Prodromal chimpanzee feces, shown to induce hepatitis in marmosets (Saginus sp.), was clarified (JA 20/8K/30 min/5C), the virus precipitated with 7% PEG 6000, harvested (JA 20/8K/30 min/5C) and resuspended. The suspensions were layered onto 5-30% linear surcrose gradients, centrifuged (SW27/25K/3 hrs/5C) and the fractions containing HAV were pooled, dialyzed and ampulized. A 1:500,000 dilution of this preparation induced hepatitis in 2/4 and seroconversion in 2/4 marmosets. A 1:50 dilution of this preparation that routinely produced hepatitis in 100% of the marmosets inoculated served as inoculum for subsequent experiments.