Record Display for the EPA National Library Catalog

RECORD NUMBER: 3 OF 5872

OLS Field Name OLS Field Data
Main Title 'Giardia' Detection in Water Systems.
Author Sauch, J. F. ;
CORP Author Health Effects Research Lab., Cincinnati, OH.
Year Published 1986
Report Number EPA/600/D-86/234;
Stock Number PB87-104493
Additional Subjects Giardia ; Water supply ; Gastro intestinal diseases ; Detection ; Immune serums ; Cysts ; Identifying ; Biological stains ; Fluorescence ; Cell morphology ; Chlorination ; Tests ; Pennsylvania ; Massachusetts ;
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB87-104493 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 11p
Abstract
A method has been developed combining immunofluorescence and phase-contrast to microscopically locate and identify Giardia cysts among particulates filtered from water samples. Selective staining of cysts with anti-Giardia antiserum and a fluorochrom enhances the contrast between cysts and other sample constituents and allows quick location of cysts by their green fluorecence. Their identity can then be confirmed with phase-contrast observation of size, shape, and internal morphology. The method was successfully tested during recent outbreaks in Pennsylvania and Massachusetts. However, when chlorinated water samples were tested, confirmation of presumptive Giardia cysts was difficult or impossible.