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RECORD NUMBER: 3504 OF 3656

OLS Field Name OLS Field Data
Main Title Use of the Microscreen Phage-Induction Assay to Assess the Genotoxicity of 14 Hazardous Industrial Wastes.
Author Houk, V. S. ; DeMarini, D. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1988
Report Number EPA/600/J-88/042;
Stock Number PB88-224373
Additional Subjects Industrial wastes ; Organic wastes ; Salmonella ; Volatile organic compounds ; Metals ; Reprints ; Hazardous wastes ; Microscreening ; Mutagenicity tests ; Virus activation ; Phage lambda
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB88-224373 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 12/29/1988
Collation 20p
Abstract
The Microscreen phage-induction assay, which quantitatively measures the induction of prophage lambda in Escherichia coli WP2s lambda, was used to test 14 crude (unfractionated) hazardous industrial waste samples for genotoxic activity in the presence and absence of metabolic activation. Eleven of the 14 wastes induced prophage, and induction was observed at concentrations as low as 0.4 picograms per ml. Comparisons between the mutagenicity of these waste samples in Salmonella and their ability to induce prophage lambda indicate that the Microscreen phage-induction assay detected genotoxic activity in all but one of the wastes that were mutagenic in Salmonella. Moreover, the Microscreen assay detected as genotoxic 5 additional wastes that were not detected in the Salmonella assay. The applicability of the Microscreen phage-induction assay for screening hazardous wastes for genotoxic activity is discussed along with some of the problems associated with screening highly toxic wastes containing toxic volatile compounds.