Record Display for the EPA National Library Catalog

RECORD NUMBER: 254 OF 1721

OLS Field Name OLS Field Data
Main Title Bioassay-Directed Fractionation of the Organic Extract of SRM 1649 Urban Air Particulate Matter.
Author Lewtas, J. ; Chuang, J. ; Nishioka, M. ; Petersen, B. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Battelle Columbus Labs., OH.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/541;
Stock Number PB91-242511
Additional Subjects Aerosols ; Particulates ; Fractionation ; Solvent extraction ; Air pollution ; Bioassay ; Urban areas ; Mutagenicity tests ; Samples ; Reprints ; SRM 1649 ; Standard reference materials
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB91-242511 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 11/26/1991
Collation 14p
Abstract
Separation of 2 grams of the methylene chloride extraction of NIST SRM 1649 (Washington, D.C. urban air particulate matter) into six compound class fractions by acid-base partitioning and silica gel column chromatography is demonstrated here. Recoveries of organic mass and Salmonella typhimurium TA98 mutagenic activity were greater than 80 percent. The fractionation method showed reproducible distribution of both mass and mutagenic activity; the compound class mass distribution results were similar to those obtained for more typical analytical fractionation of a milligram quantity of the extract mass. The most polar compound class fractions contained the greatest proportions of mutagenic activity. (Copyright (c) 1990 Gordon and Breach, Science Publishers, Inc.)