Record Display for the EPA National Library Catalog

RECORD NUMBER: 109 OF 488

OLS Field Name OLS Field Data
Main Title Determination of Mineral Fiber Concentrations in Fish Tissues.
Author Batterman, Allan R. ; Cook, Philip M. ;
CORP Author Environmental Research Lab.-Duluth, MN.
Year Published 1981
Report Number EPA-600/J-81-614;
Stock Number PB83-182980
Additional Subjects Fishes ; Mineral fibers ; Amphiboles ; Electron microscopy ; Residues ; Concentration(Composition) ; Reprints ; Lake trout ; Bioaccumulation ; Water pollution effects(Animals)
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB83-182980 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 10p
Abstract
Submicroscopic inorganic particle concentrations in tissue have not been quantitatively determined in the past because of a lack of sample preparation techniques capable of achieving the sensitivity required. The determination of whether mineral fibres in water are accumulated in aquatic organisms requires transmission electron microscope examination of bulk tissue residues rather than thin sections. The sample preparation method used for this investigation involved removal of water and organic matter by freeze-drying and low temperature ashing. Lake trout with a lifetime exposure to Lake Superior water containing amphibole fibers contained similar amphibole fibers particularly in the kidney and with low concentrations in muscle tissue. Lake trout from two locations with widely different water fiber concentrations had corresponding differences in tissue fiber concentrations. Analysis of other fish raised under laboratory conditions suggests that ingestion is the primary route for fiber accumulation.