||Microbial Transformation Rates of Azo and Triphenylmethane Dyes.
Michaels, G. B. ;
Lewis, D. L. ;
||Environmental Research Lab., Athens, GA. ;Gainesville Junior Coll., GA. Dept. of Biology.
Triphenylmethane dyes ;
Color index acid orange 6 ;
Color index basic violet 1 ;
Color index basic violet 3 ;
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Using field-collected and laboratory cultures, bacterial transformation rates for one azo and two triphenylmethane dyes were studied as a means of assessing the potential degradation of these compounds in aquatic environments. Transformation was measured as loss of color by spectrophotometry, and first- and second-order transformation rate coefficients were determined. Second-order coefficients (kb) were based on total colony-forming units (CFU) of bacteria in batch cultures. Percentages of total bacteria that transformed the dyes were determined by isolating bacterial colonies from field-collected samples and testing the isolates for the capacity to transform the dyes in dilute nutrient medium.