Record Display for the EPA National Library Catalog

RECORD NUMBER: 28 OF 307

OLS Field Name OLS Field Data
Main Title Constitutive Degradation of Trichloroethylene by an Altered Bacterium in a Gas-Phase Bioreactor.
Author Shields, M. S. ; Reagin, M. J. ; Gerger, R. R. ; Somerville, C. ; Schaubhut, R. ;
CORP Author University of West Florida, Pensacola. ;Technical Resources, Inc., Gulf Breeze, FL.;Environmental Research Lab., Gulf Breeze, FL. Office of Research and Development.;SBP Technologies, Inc., Gulf Breeze, FL.;Department of the Air Force, Washington, DC.
Publisher 1994
Year Published 1994
Report Number EPA-68-03-3479 ;USAF-F-08635-92-C-0103; EPA/600/A-94/204;
Stock Number PB95-122941
Additional Subjects Water pollution control ; Bioreactors ; Bacteria ; Waste water treatment ; E coli ; Pseudomonas ; Biodegradation ; Toluene ; Chemical analysis ; Biotechnology ; Ground water ; Aerobic conditions ; Chlorinated aliphatic hydrocarbons ; Reprints ; Ethylene/trichloro ; TOM(Toluene orth-monooxygenase) ; TCE(Trichloroethylene)
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB95-122941 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/06/1995
Collation 20p
Abstract
Pseudomonas cepacia G4 expresses a unique toluene orth-monooxygenase (Tom) that enables it to degrade toluene and trichloroethylene (TCE). Transposon mutants of G4 have been isolated that constitutively express Tom. Two fixed-film bioreactor designs were investigated for the exploitation of one such constitutive strain (G4 PR1) in the degradation of vapor-phase TCE. DNA probe analysis indicates that the genes responsible for the toluene catabolic pathway are located on a large plasmid of G4 (> or = 150 kb) termed pG4L. The authors propose that pG4L will serve as a archetype for a new class of catabolic plasmid known as Tom, which encodes an ortho-hydroxylation pathway for the degradation of benzene, toluene, o-xylene, cresols, and phenol.