||Evaluation of exposure markers : final report /
Tice, Raymond R.
||Integrated Lab. Systems, Research Triangle Park, NC.;Environmental Monitoring Systems Lab., Las Vegas, NV.;Army Biomedical Research and Development Lab., Fort Detrick, MD.
|| U.S. Environmental Protection Agency, Office of Research and Development, Environmental Monitoring Systems Laboratory,
Hazardous wastes--Physiological effect. ;
Mutagenicity tests ;
DNA damage ;
Biological markers ;
Environmental pollutants ;
In vivo analysis ;
Superfund sites ;
Chinese hamsters ;
Metabolic activation ;
In vitro analysis ;
Carcinogenicity tests ;
Hazardous wastes ;
Single gel electrophoresis ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||109 pages : illustrations, charts ; 28 cm
The primary purpose of the research has been to determine the suitability of the single cell gel (SCG) assay technique for detecting DNA damage induced by genotoxic pollutants in cells sampled from various organs of rodents and in cells sampled from humans. The major chemicals evaluated include acrylamide, trichloroethylene, and dimethylbenzanthracene. The focus of the research has been on: (1) evaluating the specificity and sensitivity of the technique by determining the magnitude and kinetics of DNA damage induced in cultured mammalian cells by a variety of genotoxic chemicals; (2) developing appropriate methods for isolating individual cells from organs of rodents; (3) evaluating the kinetics of DNA damage induced in various organs of male mice; (4) examining the applicability of the assay to peripheral blood leukocytes obtained from humans exposed to genotoxic agents; and (5) comparing the levels of DNA damage in the organs of mice collected at an EPA Superfund site. Based on the results obtained, the technique will provide, with greater sensitivity than any other method currently available, data on the induction and persistence of organ-specific levels of DNA damage resulting from environmental exposure to genotoxic pollutants.
"December 1990." "EPA/600/4-90/034." "United States Environmental Protection Agency." Includes bibliographical references. Microfiche.