||Constitutive Degradation of Trichloroethylene by an Altered Bacterium in a Gas-Phase Bioreactor.
Shields, M. S. ;
Reagin, M. J. ;
Gerger, R. R. ;
Somerville, C. ;
Schaubhut, R. ;
||University of West Florida, Pensacola. ;Technical Resources, Inc., Gulf Breeze, FL.;Environmental Research Lab., Gulf Breeze, FL. Office of Research and Development.;SBP Technologies, Inc., Gulf Breeze, FL.;Department of the Air Force, Washington, DC.
||EPA-68-03-3479 ;USAF-F-08635-92-C-0103; EPA/600/A-94/204;
Water pollution control ;
Waste water treatment ;
E coli ;
Chemical analysis ;
Ground water ;
Aerobic conditions ;
Chlorinated aliphatic hydrocarbons ;
TOM(Toluene orth-monooxygenase) ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Pseudomonas cepacia G4 expresses a unique toluene orth-monooxygenase (Tom) that enables it to degrade toluene and trichloroethylene (TCE). Transposon mutants of G4 have been isolated that constitutively express Tom. Two fixed-film bioreactor designs were investigated for the exploitation of one such constitutive strain (G4 PR1) in the degradation of vapor-phase TCE. DNA probe analysis indicates that the genes responsible for the toluene catabolic pathway are located on a large plasmid of G4 (> or = 150 kb) termed pG4L. The authors propose that pG4L will serve as a archetype for a new class of catabolic plasmid known as Tom, which encodes an ortho-hydroxylation pathway for the degradation of benzene, toluene, o-xylene, cresols, and phenol.