Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Comparison of In vivo and In vitro Methods for Assessing the Effects of Carbon Tetrachloride on the Hepatic Drug-Metabolizing Enzyme System.
Author Chadwick, R. W. ; Copeland, M. F. ; Carlson, G. P. ; Trela, B. A. ; Most, B. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC. ;Purdue Univ., Lafayette, IN. Dept. of Pharmacology and Toxicology.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/545;
Stock Number PB91-109264
Additional Subjects Toxicology ; Carbon tetrachloride ; Liver ; Chlorobenzenes ; Enzymes ; In vitro analysis ; In vivo analysis ; Metabolism ; Insecticides ; Solvents ; Reprints ; UDP glucuronosyltransferase ; Sulfotransferases ; Glutathione transferases ; Cytochrome P-450
Library Call Number Additional Info Location Last
NTIS  PB91-109264 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/04/1991
Collation 10p
The effect of a single i.p. injection of 0, 20, 200, and 1000 microliters/kg carbon tetrachloride on the activity of the hepatic drug-metabolizing enzyme system was measured in the rat by a model substrate assay, employing lindane (gamma-hexachlorocyclohexane), and by a battery of in vitro enzyme assays. The data in the study indicated that carbon tetrachloride had a biphasic influence on the phase I reactions with the lowest dose inducing a significant increase in enzyme activity while the highest dose produced significant inhibition. Significant CCl4-induced reductions in glucuronyltransferase and sulfotransferase activities were also observed while the effect on glutathione-S-transferase was ambiguous. The in vivo and in vitro assays showed good agreement.