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RECORD NUMBER: 60 OF 96

OLS Field Name OLS Field Data
Main Title Preliminary Evaluation of Results of Studies Recommended by BUA for Trichloromethane.
CORP Author Hoechst A.G., Frankfurt am Main (Germany, F.R.).;Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
Publisher 28 Jul 1988
Year Published 1988
Report Number FYI-OTS-0988-0635;
Stock Number OTS-0000635
Additional Subjects Chloroform ; Trichloromethane ; Genotoxicity ; Environmental exposure ; Mutations ; Mutagenicity ; Hamsters ; Toxicity tests ; Chromosmal aberrations ; Bone marrow ; DNA ; Genes ; Hocchst(Trademark)
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Status
NTIS  OTS-0000635 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 10/08/2007
Collation 129p
Abstract
Chloroform does not induce gene mutations in the HGPRT-Test with V79 Chinese hamster cells, either in the presence or in the absence of a metabolic activation system, under the experimental conditions described (Report: Hoechst AG No. 87.0692 of July 3, 1987). Chloroform was examined for chromosomal aberrations in bone marrow cells from Chinese hamsters in two independent in-vivo tests. In the first test, bone marrow metaphases were studied at 6, 24 and 48 hours after the administration of 40, 120 and 400 mg/kg. At 6 and 24 hours there was a slight, statistically significant increase in the aberration rate in the 400 mg/kg group. This was, however, within the range of negative control values from previous studies. In addition to this, there were isolated cases of marked DNA damage (chromosomal disintegration, chromosomal exchange figures) at dosages of 120 and 400 mg/kg. Therefore, a second study was carried out with the doses in question, i.e. 120 and 400 mg/kg. The evaluation of the results confirmed to a great extent the findings of the first study, i.e. there was also a slight significant increase in the aberration rate, which was not dose-dependent. There were also isolated occurrences of marked chromosomal damage.