||Du Pont de Nemours (E.I.) and Co., Newark, DE. Haskell Lab. for Toxicology and Industrial Medicine.; Proctor and Gamble Co., Cincinnati, OH. Ivorydale Technical Center.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
The ability of tetrafluoroethylene to induce specific locus mutations at the HGPRT locus in cultured Chinese hamster ovary (CHO) cells (CHO/HGPRT Mutagenicity Assay) was evaluated in the presence and absence of Aroclor-induced rat liver S9 metabolic activation. Based on preliminary toxicity determinations, tetrafluoroethylene, was tested at nominal concentrations of 0, 20, 40, 60, 80 and 100% for treatments with and without metabolic activation. Twenty two nonactivated cultures produced a range of 100.0 - 56.5% cell survival relative to the negative control and twenty two activated cultures produced a range of 133.4 - 49.3% relative cell survival. None of the cultures produced mutant frequencies significantly greater than the negative control with or without metabolic activation.