||Methods for measuring the toxicity and bioaccumulation of sediment-associated contaminants with freshwater invertebrates.
||Environmental Research Lab.-Duluth, MN.
|| Office of Research and Development, U.S. Environmental Protection Agency,
Marine pollution--Toxicology--United States. ;
Marine sediments--United States--Analysis. ;
Freshwater invertebrates--Effect of pollution on--United States.
Water pollution effects ;
Fresh water ;
Test methods ;
Heavy metals ;
Mathematical models ;
Hyalella azteca ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||xiii, 133 pages : illustrations ; 28 cm
The procedures are described for testing freshwater organisms in the laboratory to evaluate the toxicity or bioaccumulation of contaminants associated with whole sediments. Sediments may be collected from the field or spiked with compounds in the laboratory. Toxicity methods are outlined for two organisms, the amphipod Hyalella azteca and the midge Chironomus tentans. The toxicity tests are conducted for 10 d in 300 ml chambers containing 100 ml of sediment and 175 ml of overlying water. Overlying water is renewed daily and test organisms are fed during the toxicity tests. The endpoint in the toxicity test with H. azteca is survival and the endpoints in the toxicity test with C. tentans are survival and growth. Procedures are primarily described for testing freshwater sediments; however, estaurine sediments (up to 15%) can also be tested with H. azteca. Guidance for conducting 28-d bioaccumulation tests with the oligochaete Lumbriculus variegatus is provided in the manual.
Distributed to depository libraries in microfiche. Shipping list no.: 1994-1021-M. "June 1994." Includes bibliographical references (pages 90-100). "EPA 600/R-94/024." Microfiche.