This verification test of the Beacon Plate Kit was conducted from July 26 through August 12, 2010 at Battelle laboratories in Columbus, OH. Reference analyses by liquid chromatography tandem mass spectrometry (LC-MS/MS) were performed the week of August 16th, 2010 by the University of Nebraska Water Sciences Laboratory. The objective of this verification test was to evaluate the microcystin test kit performance in analyzing known concentrations of microcystin in ASTM International Type II deionized (DI) water and in natural recreational water samples. The technology was used to analyze a variety of water samples for the variants microcystin-LR, microcystin-LA, and microcystin-RR. Because the technology cannot distinguish individual congeners among the more than 80 microcystin variants, the samples prepared for this test were spiked with individual variants.