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RECORD NUMBER: 3 OF 5

OLS Field Name OLS Field Data
Main Title Characterization of Sulfhydryl Proteins Involved in the Maintenance of Flagellar Straightness in Hamster Spermatozoa.
Author Cornwall, G. A. ; Chang., T. S. K. ;
CORP Author Johns Hopkins Hospital, Baltimore, MD.;Health Effects Research Lab., Research Triangle Park, NC.;National Institutes of Health, Bethesda, MD.
Publisher c1990
Year Published 1990
Report Number EPA-R-812765 ;PHS-HD-22761; EPA/600/J-90/239;
Stock Number PB91-116996
Additional Subjects Proteins ; Hamsters ; Molecular weight ; Reprints ; Sulfhydryl compounds ; Sperm tail ; Sperm motility ; Dithiothreitol ; Two-dimensional polyacrylamide gel electrophoresis ; Flow cytometry
Holdings
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Status
NTIS  PB91-116996 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/04/1991
Collation 16p
Abstract
Hamster caput epididymal spermatozoa exhibit a marked 90-180 degree bend when induced to acquire progressive motility in vitro (Cornwall et al, 1988). Flagellar bending is prevented by oxidizing sperm sulfhydryl (SH) groups with diamide. In the study, the authors examined the SH proteins involved in sperm flagellar bending using two-dimensional polyacrylamide gel electrophoresis and monobromobimane, an SH-specific fluorescent dye. Proteins extracted from samples containing bent caput spermatozoa contained more reduced SH than the same proteins extracted from straight caput spermatozoa. To further characterize these sperm SH proteins, caudal epididymal spermatozoa that exhibited straight flagella were induced to undergo flagellar bending by treatment with the SH reductant dithiothreitol.