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OLS Field Name OLS Field Data
Main Title Mechanisms of Microbial Movement in Subsurface Materials.
Author Reynolds, P. J. ; Sharma, P. ; Jenneman, G. E. ; McInerney., M. J. ;
CORP Author Oklahoma Univ., Norman. Dept. of Botany and Microbiology.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Publisher c1989
Year Published 1989
Report Number EPA-R-813559; EPA/600/J-89/188;
Stock Number PB90-140682
Additional Subjects Escherichia coli ; Penetration tests ; Subsoil ; Graphs(Charts) ; Galactose ; Growth ; Porosity ; Nutrients ; Reprints ; Motility ; Chemotaxis ; Culture media
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-140682 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/15/1990
Collation 9p
Abstract
The biological factors important in the penetration of Escherichia coli through anaerobic, nutrient-saturated, Ottawa sand-packed cores were studied under static conditions. In cores saturated with galactose-peptone medium, motile strains of E. coli penetrated four times faster than mutants defective only in flagellar synthesis. Studies with motile nonchemotactic and chemotactic microbes indicated that chemotaxis may not be required for bacterial penetration through unconsolidated porous media. Studies using motile and nonmotile E. coli strains together with their respective isogenic non-gas-producing mutants indicated that gas production may be an important mechanism for the movement of non-motile bacteria through such media, but has little effect on the penetration rate of motile organisms. Results obtained with motile strains implied that the penetration rate for motile bacteria through unconsolidated porous media is regulated by the in situ bacterial growth rate. A sigmoidal relationship was found between the specific growth rates of all of the motile bacteria used in the study and the penetration rates through cores saturated with galactose-peptone medium. (Copyright (c) 1989 American Society for Microbiology.)