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RECORD NUMBER: 2 OF 7

OLS Field Name OLS Field Data
Main Title Novel Methods for Tracking 'Agrobacterium Radiobacter' k84 and Plasmid-Borne Genes in Agricultural Ecosystems.
Author Moore, L. W. ; Loper, J. ; Stockwell, V. ; Kawalek, M. ;
CORP Author Oregon State Univ., Corvallis. Dept. of Botany and Plant Pathology. ;Agricultural Research Service, Corvallis, OR. Horticultural Crops Research Lab.;Corvallis Environmental Research Lab., OR.
Publisher Jul 91
Year Published 1991
Report Number EPA/600/3-91/042;
Stock Number PB91-216374
Additional Subjects Soil microbiology ; Plasmids ; Bacterial genes ; Terrestrial ecosystems ; Field tests ; Genetic engineering ; DNA probes ; Mutations ; Microbial drug resistance ; Virulence ; Streptomycin ; Rifampicin ; Southern blotting ; Bioassay ; Genetic conjugation ; Agrobacterium radiobacter ; Opines
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB91-216374 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/26/1991
Collation 118p
Abstract
Ecological studies of microbes introduced into the environment have been limited by the lack of sensitive and specific methods for detection and identification of released organisms among the myriad of other microbes present in nature. Similar difficulties exist for detection of DNA transfer from a released organism to other microbes in the environment. Techniques generating viable counts of specific microbes from environmental samples are among the best available for ecological studies of microorganisms released into the environment. Antiserum that is specific to certain species or strains of bacteria from environmental samples by immunoisolation techniques. Immunoisolation can detect a few cells of certain bacterial pathogens in a milliliter of seed extract. The application of immunoisolation and viable count methods to detection of released microbes in soil or on plant roots was an objective of the study.