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RECORD NUMBER: 3 OF 13

OLS Field Name OLS Field Data
Main Title Changes in Accessibility of DNA to Various Fluorochromes during Spermatogenesis.
Author Evenson, D. ; Darzynkiewicz, Z. ; Jost, L. ; Janca, F. ; Ballachey, B. ;
CORP Author South Dakota State Univ., Brookings. Dept. of Chemistry. ;Sloan-Kettering Inst. for Cancer Research, New York. Lab. of Investigative Cytology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA/600/J-86/163;
Stock Number PB87-114609
Additional Subjects Deoxyribonucleic acid ; Flow cytometry ; Reprints ; Fluorochromes ; Spermatogenesis
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB87-114609 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 11p
Abstract
Accessibility of mouse testicular and vas deferens (vas) sperm cell DNA to acridine orange, propidium iodide, ellipticine, Hoechst 33342, mithramycin, chromomycin A3, 4'6-diamidino-2-phenylindole (DAPI), and 7-amino-actinomycin D (7-amino-AMD) was determined by flow cytometry. Permealized cells were either stained directly or after pretreatment with 0.06 N HCI. For histone-containing tetraploid, diploid, and round spermatid cells, HCI extraction of nuclear proteins caused an approximately sixfold increase of 7-amino-AMD stainability but had no significant effect on DAPI stainability. For these same cell types, the stainability with other intercalating (acridine orange, propidium iodide, ellipticine) and externally binding (Hoechst 33342, mithramycin, chromomycin A3) dyes was increased by 1.6- to 4.0-fold after HCI treatment. In sharp contrast, HCI treatment of vas sperm did not increase the staining level of 7-amino-AMD, DAPI, or propidium iodide but did increase the staining level for the other intercalating dyes (1.3- to 1.5-fold) and external dyes (1.3- to 1.9-fold).