Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Dose Dependence of Acetylcholinesterase Activity in Neuroblastoma Cells Exposed to Modulated Radio-Frequency Electromagnetic Radiation.
Author Dutta, S. K. ; Das, K. ; Ghosh, B. ; Blackman, C. F. ;
CORP Author Howard Univ., Washington, DC. Dept. of Botany.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher 1992
Year Published 1992
Report Number EPA-R-812100 ;EPA-R-814126; EPA/600/J-94/022;
Stock Number PB94-137320
Additional Subjects Acetylcholinesterase ; Neuroblastoma ; Electromagnetic radiation ; Cultured tumor cells ; Calcium ; Dose-response relationships ; Glioma ; Somatic cell hybrids ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB94-137320 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 05/14/1994
Collation 10p
Radio-frequency electromagnetic radiation (RFR) at 915 and at 147 MHz, when sinusoidally amplitude modulated (AM) at 16 Hz, has been shown to enhance release of calcium ions from neuroblastoma cells in culture. The dose-response relation is unusual, consisting of two power-density 'windows' in which enhanced efflux occurs, separated by power-density regions in which no effect is observed. To explore the physiological importance of these findings, we have examined the impact of RFR exposure on a membrane-bound enzyme, acetylcholinesterase (AChE), which is intimately involved with the acetylcholine (ACh) neurotransmitter system. Neuroblastoma cells (NG108), exposed for 30 min to 147-MHz radiation, AM at 16 Hz, demonstrated enhanced AChE activity, as assayed by a procedure using (14)C-labeled ACh. Enhanced activity was observed within a time window between 7.0 and 7.5 h after the cells were plated and only when the exposure occurred at power densities identified in a previous report as being effective for altering the release of calcium ions. Thus RFR affects both calcium-ion release and AChE activity in nervous system-derived cells in culture in a common dose-dependent manner. (Copyright (c) 1992 Wiley-Liss, Inc.)