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RECORD NUMBER: 32 OF 165

OLS Field Name OLS Field Data
Main Title Comparative In vitro Percutaneous Absorption of p-Substituted Phenols through Rat Skin Using Static and Flow-Through Diffusion Systems.
Author Hughes, M. F. ; Shrivastava, S. P. ; Fisher, H. L. ; Hall, L. L. ;
CORP Author ManTech Environmental Technology, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Environmental Toxicology Div.
Publisher cMay 93
Year Published 1993
Report Number EPA-68-02-4450; EPA/600/J-93/416;
Stock Number PB94-101573
Additional Subjects Phenols ; Skin(Anatomy) ; Dermal absorption ; Comparison ; In vitro analysis ; Diffusion ; Rats ; Graphs(Charts) ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB94-101573 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 02/27/1994
Collation 8p
Abstract
The objective of this study was to determine the in vitro percutaneous absorption of (14)C-phenol and eight p-substituted derivatives and to examine the variability of this data. Two diffusion systems, the static and flow-through, were used. Clipped dorsal skin was removed from female F344 rats (90 days old), dermatomed to a thickness of approximately 350 micrometer, placed in a diffusion cell and treated with chemical (4 microgram/sq cm, in ethanol). The cumulative absorption at 72 hr for the phenols in both systems ranged from 15.4 to 97.6%. Greater than 70% of the chemical was absorbed in both systems when the chemical had a log P value between 1.4-3.5. Significant differences in percent absorption between the two diffusion systems were observed with 5 of the compounds. Absorption of acetamidophenol, chlorophenol and cyanophenol were significantly lower in the static system. Absorption of phenol and heptyloxyphenol were significantly lower in the flow-through system. The most variable absorption data in both systems were with the phenols that were absorbed the least (70%). These same chemicals were also on the extremes of the log P scale for the phenols examined. Controlling for the variability of in vitro percutaneous absorption of chemicals is necessary when using the data for human risk assessment.