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OLS Field Name OLS Field Data
Main Title Fractionation of mutagens from municipal sludge and wastewater /
Author Wright, Cherylyn W. ; Stewart, D. L. ; Weimer, W. C.
Other Authors
Author Title of a Work
Stewart, Dorothy L.
Weimer, Walter C.
CORP Author Battelle Pacific Northwest Labs., Richland, WA.;Health Effects Research Lab., Cincinnati, OH.
Publisher U.S. Environmental Protection Agency, Health Effects Research Laboratory,
Year Published 1989
Report Number EPA-CR-812051; EPA/600/1-89/001
Stock Number PB89-161491
Subjects Mutagenesis. ; Sewage sludge--Texas--Analysis. ; Sewage sludge--Washington (State)--Analysis. ; Sewage sludge--Analysis.
Additional Subjects Microbiology ; Mutagens ; Sludge disposal ; Sewage disposal ; Sampling ; Solvent extraction ; Bioassay ; Pentane ; Chromatographic analysis ; Salmonella ; Methylene chloride ; Carbinols ; Mass spectroscopy ; Evaluation
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB89-161491 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 01/01/1988
Collation 69 pages ; 28 cm
Abstract
There are potential environmental concerns from the disposal of municipal wastewater effluents and sewage treatment plant sludges. The report summarizes the microbial mutagenic evaluation of 13 sewage sludge samples from various locations in Texas and Washington state. The sewage sludge samples were air-dried followed by sequential Soxhlet extraction with pentane, methylene chloride, and methanol. The organic extracts from three of the samples were further fractionated by normal phase high-pressure liquid chromatography (HPLC). The obtained extracts and fractions were bioassayed for microbial mutagenic response using the standard histidine reversion assay with Salmonella typhimurium strains TA98 and TA100, both with and without S9 metabolic activation. Extracts and fractions were chemically analyzed by high resolution gas chromatography (GC) using a variety of element-specific detectors, gas chromatography/mass spectrometry (GC/MS), and liquid chromatography/mass spectrometry (LC/MS).
Notes
Caption title. "February 1989." "EPA/600/1-89/001." Microfiche.