||Fractionation of mutagens from municipal sludge and wastewater /
Wright, Cherylyn W. ;
Stewart, D. L. ;
Weimer, W. C.
||Battelle Pacific Northwest Labs., Richland, WA.;Health Effects Research Lab., Cincinnati, OH.
|| U.S. Environmental Protection Agency, Health Effects Research Laboratory,
Sewage sludge--Texas--Analysis. ;
Sewage sludge--Washington (State)--Analysis. ;
Sludge disposal ;
Sewage disposal ;
Solvent extraction ;
Chromatographic analysis ;
Methylene chloride ;
Mass spectroscopy ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||69 pages ; 28 cm
There are potential environmental concerns from the disposal of municipal wastewater effluents and sewage treatment plant sludges. The report summarizes the microbial mutagenic evaluation of 13 sewage sludge samples from various locations in Texas and Washington state. The sewage sludge samples were air-dried followed by sequential Soxhlet extraction with pentane, methylene chloride, and methanol. The organic extracts from three of the samples were further fractionated by normal phase high-pressure liquid chromatography (HPLC). The obtained extracts and fractions were bioassayed for microbial mutagenic response using the standard histidine reversion assay with Salmonella typhimurium strains TA98 and TA100, both with and without S9 metabolic activation. Extracts and fractions were chemically analyzed by high resolution gas chromatography (GC) using a variety of element-specific detectors, gas chromatography/mass spectrometry (GC/MS), and liquid chromatography/mass spectrometry (LC/MS).
Caption title. "February 1989." "EPA/600/1-89/001." Microfiche.