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RECORD NUMBER: 4 OF 4

OLS Field Name OLS Field Data
Main Title Selection Kinetics during Serial Cell Culture Passage of Mixtures of Wild-Type 'Autographa californica' Nuclear Polyhedrosis Virus and Its Recombinant Ac360-Beta-gal.
Author Huang, Y. S. ; Bobseine, K. L. ; Setzer, R. W. ; Kawanishi., C. Y. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-92/015;
Stock Number PB92-143874
Additional Subjects Baculoviridae ; Genetic recombination ; Selection(Genetics) ; Kinetics ; Cultured cells ; Genetic engineering ; Electron microscopy ; Plaque assay ; Phenotype ; Virulence ; Reprints ; Autographa californica
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB92-143874 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/28/1992
Collation 10p
Abstract
Detailed analysis of the selection process in serial co-infections of cell cultures by wild type Autographa californica nuclear polyhedrosis virus, AcNPV/E2, and Ac360-beta-gal, a genetically engineered strain, shows that the unaltered strain was clearly dominant even when it began as the minority component in the inoculum. A method of calculating a selection coefficient that quantifies the relative advantage of one strain of virus over the other under specific culture conditions is described. Calculated selection coefficients were relatively homogeneous and almost exclusively favored the progenitor. Selection pressure was uninfluenced by the relative proportions of the two strains in the population. Unexpected high frequencies of mixed phenotype plaques were observed during infectivity titrations of media from early serial passages of coinfected cultures. Statistical evaluation implicates some nonheritable combinational phenomenon. Virus plated from mixed phenotype plaques show high segregation of phenotypes implying that genetic recombination does not contribute in a major way to the high mixed phenotype frequencies.